TACF@ICGEB ICGEB:: www.icgeb.res.in

Tuberculosis Aerosol Challenge Facility

A national facility hosted at ICGEB, New Delhi and supported by Department of Biotechnology,Government of India for conducting cutting edge research on tuberculosis in India.

PI: Dr Dinakar M. Salunke & CO-PI: Dr Dhiraj Kumar

IVC for Guinea pigs

IVC for mice

FACS Sorter facility

Madison Aerosol Exposure Chamber

FACILITY:

Aerosol challenge facility for mice and guinea pigs

In vitro experiments with Mycobacterium tuberculosis

Ex vivo experiments with immune cells

FACS Sorter (BD FACS ARIA Fusion, BSL3)

High content screening facility (BSL3)

Aerosol challenge facility for mice and guinea pigs

USERS : Current and Past

THSTI, Faridabad
NII, New Delhi
JNU, New Delhi
Delhi University, New Delhi
CDFD, Hyderabad
PGI, Chandigarh
IMTECH, Chandigarh
IVRI, Izatnagar
IGIB, New Delhi
AIIMS, New Delhi

Publication:

1. Manitosh Pandey, Alok Kumar Singh, Ritesh Thakare, Sakshi Talwar, Pratiksha Karaulia, Arunava Das Gupta, Sidharth Chopra & Amit Kumar Pandey 2017: Diphenyleneiodonium chloride (DPIC) displays broad-spectrum bactericidal activity. Scientific Reports 7:11521
2. Saqib Kidwai, Chan-Yong Park, Shradha Mawatwal, Prabhakar Tiwari, Myung Geun Jung, Tannu Priya Gosain, Pradeep Kumar, David Alland, Sandeep Kumar, Avinash Bajaj, Yun-Kyung Hwang, Chang Sik Song, Rohan Dhiman, Young Lee & Ramandeep Singh 2017: The dual mechanism of action of 5-Nitro- 1,10-phenanthroline against Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 61(11): e00969-17.
3. Mehak Zahoor Khan, Ashima Bhaskar, Sandeep Upadhyay, Pooja Kumari, Raju S. Rajmani, Preeti Jain, Amit Singh, Dhiraj Kumar, Neel Sarovar Bhavesh & Vinay Kumar Nandicoori 2017: Protein kinase G confers survival advantage to Mycobacterium tuberculosis during latency-like conditions. Journal of Biological Chemistry 292, 16093-16108
4. Haroon Kalam, Mary F. Fontana, Dhiraj Kumar 2017: Alternate splicing of transcripts shape macrophage response to Mycobacterium tuberculosis infection. PLoS Pathogen 13(3): e1006236.
5. Garima Arora, Deepika Chaudhary, Saqib Kidwai, Deepak Sharma & Ramandeep Singh 2018: CitE Enzymes Are Essential for Mycobacterium tuberculosis to Establish Infection in Macrophages and Guinea Pigs. Front. Cell. Infect. Microbiol. 8:385
6. Sakshi Agarwal, Prabhakar Tiwari, Amar Deep, Saqib Kidwai, Shamba Gupta, Krishan Gopal Thakur & Ramandeep Singh 2018: System-Wide Analysis Unravels the Differential Regulation and In Vivo Essentiality of Virulence-Associated Proteins B and C Toxin-Antitoxin Systems of Mycobacterium tuberculosis. The Journal of Infectious Diseases 217(11): 1809-1820.
7. Haroon Kalam, Kartikeya Singh, Komal Chauhan, Mary F. Fontana & Dhiraj Kumar 2018: Alternate splicing of transcripts upon Mycobacterium tuberculosis infection impacts the expression of functional protein domains. IUBMB Life
8. Divya Arora, Yogesh Chawla, Basanti Malakar, Archana Singh & Vinay Kumar Nandicoori 2018: The transpeptidase PbpA and non-canonical transglycosylase RodA of Mycobacterium tuberculosis play important roles in regulating bacterial cell lengths. Journal of Biological Chemistry doi: 10.1074/jbc.M117.811190.
9. Sanjeev Kumar Shukla,Shubhra Shukla, Rehan Khan, Anuj Ahuja, Lakshya Veer Singh, Nitin M. Kamble, Anupam Dhasmana, Naveen Sharma, Bipin Chand, Ajay Vir Singh 2018: Pathway analysis of differentially expressed genes in Mycobacterium bovis challenged bovine macrophages. Microbial Pathogenesis.115:343-352.

For animal experiments:

Get IBSC and IAEC approval from the parent Institute/University/Company
Apply for ICGEB IAEC approval in a prescribed format (available on request)
Once approved, dates are allotted as per availability

For In vitro/ Ex vivo experiments:

Get IBSC approval from the parent Institute/University/Company
Submit the request for In vitro/ Ex vivo experiments to TACF In-Charge with a list of experiments
Time slots allotted based on availability

★ ICGEB-TACF remains open for all users during working hours of week days (except for national holidays).

★ Access is controlled through an electronic/biometric access control system, staffs at TACF help accessing the lab areas.

$CHARGES

Revised charges for experiments/access at TACF (W. E. F. January 2017):

Mice	INR 75/animal/week
Guinea pigs	INR 400/animal/week
In vitro/Ex vivo experiments	INR 5000/week

Payments to be made at:

By Demand draft in favor of ICGEB