Go to the abstract in the NAR 2003 Database Issue.
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Contact vbrendel@iastate.edu
Zea mays DataBase (ZmDB) is a repository and analysis tool for sequence, expression, and phenotype data of the major crop plant maize. The data accessible in ZmDB are mostly generated in a large collaborative project of maize gene discovery, sequencing, and phenotypic analysis using a transposon tagging strategy and expressed sequence tag (EST) sequencing. More than 73,000 ESTs currently have been assembled to a tentative set of unique genes consisting of approximately 22,500 consensus cDNAs. Shortly to be added will be up to 150,000 genomic sequence fragments flanking the transposon insertions. Phenotypic screens are conducted on all mutant plants. An associated phenotype database identifies each plant and will eventually cross-reference the EST and genomic sequence data. Microarray technology is used to document mRNA expression profiles in different tissues. In total, database search tools, convenient links to external databases, and novel sequence analysis programs for spliced alignment are provided and together serve as an efficient protocol for gene discovery by sequence inspection. ZmDB also provides web-based ordering of materials generated in the project, including EST and genomic DNA clones, seeds of mutant plants, and microarrays of amplified EST and genomic DNA sequences.
Gai, X., Lal, S., Xing, L., Brendel, V. and Walbot, V. (2000) Gene discovery using the maize genome database ZmDB. Nucl. Acids Res. 28: 94-96.
Category Genomic Databases
Go to the abstract in the NAR 2003 Database Issue.