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FLAGdb/FST

http://genoplante-info.infobiogen.fr

Samson, F.1, Brunaud, V.1, Balzergue, S.1, Dubreucq, B.2, Lepiniec, L.2, Pelletier, G.3, Caboche, M.1, Lecharny, A.1

1URGV, INRA, FRE CNRS, 2, rue Gaston Crémieux, F-91000 Evry, France.
2Laboratoire de Biologie des Semences, INRA, F-78026, Versailles, France.
3Station de Génétique et Amélioration des Plantes, INRA, F-78026, Versailles, France.

Contact   lecharny@ibp.u-psud.fr


Database Description

A large collection of T-DNA insertion transformants of Arabidopsis thaliana has been generated, at Institute of Agronomic Research, Versailles, France. The molecular characterisation of the insertion sites is currently done by sequencing genome regions flanking the inserted T-DNA (FST) (Balzergue et al., 2001). The pseudo-chromosomes of A. thaliana (TIGR, ftp://ftp.tigr.org/pub/data/a_thaliana/ath1/PSEUDOCHROMOSOMES/) provides the framework for organising FSTs in a genome oriented database, FLAGdb/FST (1), http://genoplante-info.infobiogen.fr). The main scope of FLAGdb/FST is to help biologists to find the FSTs that interrupt the genes they are interested in. Requests are made using BLAST programs to locate the genomic position of a query sequence. The response delivered by FLAGdb/FST is a graphical representation of the putative FSTs and of predicted genes in a 20 kb long region.

Acknowledgements

We thank the staffs of GENOPLANTE-INFO and INFOBIOGEN for their help and cooperation in maintaining FLAGdb/FST on their site. This work was supported by the French Génoplante Program.

REFERENCES

Balzergue S., Dubreucq B., Chauvin S., Le-Clainche I., Le Boulaire F., deRose R., Samson F., Biaudet
V., Lecharny A., Cruaud C., Weissenbach J., Caboche M. and Lepiniec L. (2001) Improved PCR-Walking for large-scale isolation of plant T-DNA borders. BioTechniques, 30, 496-504.
Samson F., Brunaud V., Balzergue S., Dubreucq B., Lepiniec L., Pelletier G., Caboche M. and Lecharny A. (2002) FLAGdb/FST: a database of mapped flanking insertion sites (FSTs) of Arabidopsis thaliana T-DNA transformants. Nucleic Acids Res. Vol. 30, No. 1 94-97

Category   Mutation Databases

Go to the abstract in the NAR 2002 Database Issue.

 

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