Go to the abstract in the NAR 2002 Database Issue.
 |
 |
|||||||
|
||||||||
Kumar, A.1, Cheung, K.-H.2, Tosches, N.2, Masiar, P.2, Liu, Y.2, Miller, P.2, Snyder, M.1
1Dept. of Mol., Cell., and Dev. Biology Yale University PO Box 208103 New Haven, CT 06520-8103, USA
2Center for Medical Informatics Yale University School of Medicine 333 Cedar Street PO Box 208009 New Haven, CT 06520-8009, USA
Contact anuj.kumar@yale.edu
Using a novel multipurpose mini-transposon, we have generated a collection of defined mutant alleles for the analysis of disruption phenotypes, protein localization, and gene expression in Saccharomyces cerevisiae. To catalog this unique data set, we have developed TRIPLES, a Web-accessible database of TRansposon-Insertion Phenotypes, Localization, and Expression in Saccharomyces. Encompassing over 500,000 data points, TRIPLES provides convenient access to information from nearly 28,000 transposon-mutagenized yeast strains; complete data reports of each strain may be viewed in table format, or if desired, downloaded as tab-delimited text files. Protein localization data within TRIPLES has been supplemented with over 500 fluorescent micrographs of staining patterns observed by indirect immunofluorescence analysis of indicated epitope-tagged proteins. The TRIPLES web site also provides access to similar micrographs of an additional 2,000 immunolocalized yeast proteins, generated as part of our large-scale analysis defining the subcellular localization of the yeast proteome. In total, TRIPLES now represents one of the largest on-line visual libraries of yeast protein localization to date. In addition, each report within TRIPLES contains external links to corresponding entries within the Saccharomyces Genome Database (SGD), and International Nucleic Acid Sequence Data Library (GenBank). Unlike other yeast databases, TRIPLES provides on-line order forms linked to each clone report; users may immediately request any desired strain free-of-charge by submitting a completed form. In addition to presenting a wealth of information for over 3,750 open reading frames, TRIPLES constitutes an important medium for the distribution of useful reagents throughout the yeast scientific community. Maintained by Michael Snyder's lab at Yale University, TRIPLES may be accessed at http://ygac.med.yale.edu.
To facilitate intuitive interpretation of our data, users may now browse records within TRIPLES by means of a convenient graphic map accessible through the 'Display Insertion Map' link incorporated into our basic search forms. The resulting map displays all transposon insertions (represented as flags) within the chosen gene; composite records corresponding to each insertion may be viewed by simply clicking on an insertion 'flag' of interest.
This work is supported by NIH grant R01-CA77808 to M.S. A.K. is supported by a post-doctoral fellowship from the American Cancer Society.
Category Gene Expression
Go to the abstract in the NAR 2002 Database Issue.